The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery Studies

The chicken embryo and the blood-vessel rich chorioallantoic membrane (CAM) is a valuable in vivo model to investigate biomedical processes, new ultrasound pulsing schemes, or novel transducers for contrast-enhanced imaging microbubble-mediated drug delivery. reasons this are accessibility of vessel network CAM as well low costs model. An important step get access vessels take egg content out eggshell. In protocol, three methods taking eggshell between day 5 8 incubation described thus allowing embryos develop inside up these days. only require simple tools equipment yield higher survival success rate 90% 5-day, 75% 6-day, 50% 7-day, 60% 8-day old incubated eggs comparison ex ovo cultured (~50%). protocol also describes how inject cavitation nuclei, such microbubbles, into vascular system, separate containing from rest optically transparent studies, use variety short-term experiments. extremely relevant protocols, contrast agents, schemes imaging, unravel mechanisms ultrasound-mediated